三种病毒感染所致淋巴细胞及T细胞亚群改变的分析

摘要

目的 观察分析EB病毒(EBV)、巨细胞病毒(CMV)和H1N1病毒感染者外周血白细胞(WBC)数量和形态变化,比较不同类型异型淋巴细胞分布及其与T细胞亚群变化的关系.方法 对3种不同病毒感染者进行WBC计数和分类计数以及异型淋巴细胞观察.对异型淋巴细胞增高者进行T细胞亚群测定并进行比较分析,并与同期健康体检者(对照组)进行比较.结果 3种病毒感染者的外周WBC数量和中性粒细胞、淋巴细胞分类以及异型淋巴细胞计数与对照组比较,差异均有统计学意义(P<0.05);3种病毒感染者的异型淋巴细胞分布均以Ⅱ型为主,但CMV与H1N1病毒感染者的异型淋巴细胞分布比EBV感染者离散程度高;3种病毒感染者CD3+、CD4+T细胞数量与对照组比较,差异均有统计学意义(P<0.05);3种病毒感染者与对照组CD8+T细胞数量比较,差异均有统计学意义(P<0.05),且EBV与CMV、H1N1组间比较,差异有统计学意义(P<0.05).结论 3种病毒感染者异型淋巴细胞都有不同程度增高,但以EBV感染者增高明显;3种病毒感染者都有不同程度免疫功能紊乱,但以EBV感染者最明显.外周血异型淋巴细胞增高与机体免疫系统反应程度有关.%Objective To Observe and analyze quantity and morphological changes of peripheral blood leukocyte in patients infected with the EBV,CM V or H1N1 viruses,compare the distribution of atypical lymphocytes and its changes in correlation with T lymphocyte subsets .Methods WBC count and classification and atypical lymphocyte observation were performed in patients infer -ted with the three virus .For patients whose atypical lymphocytes increased determination and comparative analysis of T -cell subsets were conducted .Results Compared with the control group,peripheral white Wood cell count,neutrophils,lymphocytes,and atypical lymphocyte classification count in patient with three kinds of viral infection were significantly different (P^-0 .05) .The distribution of atypical lymphocytes in patient infected with three viruses were mainly type II,but the distribution of atypical lymphocytes in patient infected with CMV AND the H1N1 virus were more discrete than with EBV .There were significant difference (P<0 .05) in CD3 and CD4 T cell between patients infected with three kinds of virus and control group .It was the same(P<0 .05) in CD8 T cell between patients infected with three kinds of virus and control group,in which there was significant diffierence in comparison between EBV and CMV,H1N1 virus(P<0 .05) .Conclusion The atypical lymphocytes caused by three kinds virus infection could increase to varying degrees,but in patient with EBV infection could increase more significantly .And patients infected with three kinds of virus might have a different degree of immune dysfunction,but there could be a higher degree with EBV infection than the other two infections .There could be a correlation between the increased count of atypical lymphocytes in peripheral Wood and the degree of immune system response .