Objective To investigate the application value of amniotic cell culture and karyotype analysis in prenatal diagnosis . Methods 1 016 pregnant women in second trimester were subject to amniocentesis under the guidance of B-type ultrasonic inspec-tion ,and the cell culture and karyotype analysis were performed on the amniotic fluid which had been drawn out .Results Among 1 016 pregnant women ,1 011(99 .5% ) succeeded in the first operation of amniocentesis and cell culture .The detection rate of fetal chromosomal abnormal karyotype was 10 .3% (105/1 016) ,in which 8 .3% (85/1 016) of structural abnormality and 2 .0%(20/1 016) of quantity abnormality .856 cases were received follow-up .Conclusion Amniotic fluid cell culture and karyotype analy-sis is a safe and reliable method for prenatal diagnosis .%目的：探讨羊水细胞培养和染色体核型分析在产前诊断中的的应用价值。方法对1016例孕中期孕妇，在B型超声波检查的监护下行羊膜腔穿刺术，对抽出的羊水进行细胞培养及染色体核型分析。结果 1016例孕妇中，一次性羊水穿刺、细胞培养成功1011例（99．5％）。胎儿染色体异常核型检出率为10．3％（105/1016），其中，结构异常8．3％（85/1016）；数目异常2．0％（20/1016）。856例接受随访。结论羊水细胞培养及染色体核型分析是安全、可靠的产前诊断方法。
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