混合型固相萃取技术及毛细管电泳检测血浆和尿液中4种喹诺酮类药物∗

摘要

Objective To develop a new method for the rapid determination of lomefloxacin,gatifloxacin,ciprofloxacin and oflox-acin in plasm and urine by solid phase extraction(SPE)and capillary electrophoresis.Methods The capillary was fused silica capil-lary with id/od of 75/365 μm and effective/total length of 40/47 cm.The running buffer was 40 mmol/L borate buffer at pH 9.0. Separation voltage was 13 kV.Temperature was 20 ℃.Detection wave-length was set at 280 nm.The sample was analyzed after the pretreatment of SPE.Results The analysis of lomefloxacin,gatifloxacin,ciprofloxacin and ofloxacin was completed in 6 minutes with satisfied accuracy and precision.Good linearity was found within the range of 1-40 μg/mL,and the r was 0.998 7,0.997 6, 0.998 3 and 0.994 2 respectively.The recoveries of four quinolones in plasm and urine ranged from 80.1% to 107.6%,and the rel-ative standard deviations(RSD)ranged from 2.1% to 6.2%.Conclusion This method is fast,simple,precise and it might be feasi-ble for the determination of lomefloxacin,gatifloxacin,ciprofloxacin and ofloxacin in plasm and urine samples.%目的：建立固相萃取及毛细管电泳法快速检测血浆和尿液中喹诺酮类药物洛美沙星、加替沙星、环丙沙星和氧氟沙星的方法。方法毛细管为熔融石英毛细管(75/365μm,40/47 cm),运行缓冲液为40 mmol/L 硼砂缓冲液(pH 9.0),电压13 kV,柱温为20℃,检测波长280 nm。样品经固相萃取后进样分析。结果4种喹诺酮类药物在6 min 内完全分离。在1~40μg/mL 浓度范围内,洛美沙星、加替沙星、环丙沙星和氧氟沙星峰面积和浓度的线性关系良好,相关系数 r 分别为0.9987、0.9976、0.9983和0.9942。尿液和血浆中4种喹诺酮类药物加标回收率为80.1%~107.6%,精密度相对标准差(RSD)为2.1%~6.2%。结论该方法具有快速、简便、准确度和精密度高等特点,适用于血浆和尿液中洛美沙星、加替沙星、环丙沙星和氧氟沙星水平的快速检测。