免疫分型在单株丙球蛋白血症中的应用评估

摘要

Objective To investigate the clinical value of immunophenotyping and quantitative analysis of serum immunoglobulin in the diagnosis and typing of monoclonal gammaglobulinemia.Methods 118 serum samples were collected from the patients with monoclonal gammaglobulinemia and performed the serum protein electrophoresis(SPE),serum immunofixation(IF)electrophoresis, immunoglobulins(Ig)quantitation and serum light chain κ,λdetection.The analysis was conducted aiming at the patients with im-munophenotyping positive and the Ig quantitation negative.Results 56 cases showed immunophenotyping positive and the immuno-globulin quantitation negative,among them,6 cases were free light chain type (κlight chain in 2 cases andλlight chain in 4 cases), 1 case was the non-secretion type;62 cases showed immunophenotyping positive and the Ig quantitation positive.Conclusion The IF technique has an important significance for the diagnosis and typing of monoclonal gammaglobulinemia,its diagnosis can be com-bined with the other laboratory tests of serum Ig and light chain quantitative detection.%目的：探讨免疫分型、血清免疫球蛋白的定量检测在单株丙球蛋白血症诊断中的价值。方法收集118例单株丙球蛋白血症患者进行血清蛋白电泳（SPE）、免疫固定电泳（IF）、免疫球蛋白（Ig）定量及血清轻链（κ、λ）检测。针对免疫分型阳性、Ig定量阴性患者进行分析。结果免疫分型阳性、Ig 定量阴性患者56例，其中游离轻链型6例（κ轻链型2例，λ轻链型4例），未分泌型1例；免疫分型阳性、Ig 定量阳性的患者62例。结论 IF 技术对单株丙球蛋白血症的诊断及分型具有重要意义，可以结合血清 Ig 及轻链的定量检测等其他实验室诊断。