目的：探讨β‐catenin在铜绿假单胞菌角膜炎中发挥的作用及其调控机制。方法 Western‐blot检测β‐catenin慢病毒应用的效果，临床评分观察疾病进展；Real‐time聚合酶链式反应（PCR）检测炎性因子和抗菌肽的表达；平板细菌计数检测角膜细菌清除情况。结果β‐catenin延缓角膜炎的疾病进展；随后实验发现β‐catenin抑制促炎因子的分泌，并上调抗菌肽CRAM P表达，促进细菌清除。结论β‐catenin通过调节炎性反应并促进细菌清除，在铜绿假单胞菌角膜炎中发挥角膜保护作用。%Objective To explore the role of β‐catenin in Pseudomonas aeruginosa(PA) keratitis and its regulatory mechanism . Methods Western‐blot assay was used to detect the efficacy of β‐catenin lentivirus application .The clinical score was used to ob‐serve the disease progression ;the expression of inflammatory cytokines and antimicrobial peptides were examined by using real‐time PCR;the corneal bacterial clearance was detected by using the plate bacterial count .Results β‐catenin delayed the progression of keratitis ;then the following experiment found that β‐catenin suppressed the secretion of pro‐inflammatory cytokines ,up‐regulated the antibacterial peptide cathelicidin‐related antimicrobial peptide(CRAMP) expression and promoted the bacterial clearance .Con‐clusion β‐catenin plays the corneal protective effect in PA keratitis by regulating inflammatory reactions and promoting the bacteri‐al clearance .
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