Objective To investigate the infection and distribution situations of diarrheogenic Escherichia coli among food practi‐tioners in Changsha City and to understand the carrying status of its virulence genes .Methods Totally 258 food practitioners from 9 units of Changsha City were extracted as the research subjects .Diarrheogenic Escherichia coli was detected by adopting the real time fluorescence multiple PCR .Results The infection rate of diarrheogenic Escherichia coli was 15 .5% (40/258) ,in which EAEC accounted for 10 .9% (28/258) ,EPEC for 3 .1% (8/258) and EHEC for 1 .6% (4/258) respectively ,EIEC and ETEC were not found .Among 28 strains of EAEC ,26 strains carried uidA gene ,13 strains carried aggR+pic gene and 27 strains carried astA gene , the carrying rates were 92 .9% ,46 .4% and 96 .4% respectively ;eight strains of EPEC all carried eae and uidA genes ;among 4 strains of EHEC ,3 strains carried eae gene ,moreover all 4 strains carried stx1+ stx2 gene .Conclusion Establishing a mechanism for continuously monitoring diarrheogenic Escherichia coli and deeply studying its molecular epidemiologic theory not only provide the essential data for the supervisory departments formulating and evaluating the public health measures ,but also has an important significance for evaluating the food safety status ,which can effectively control infectious source and protect the people′s health .%目的：了解长沙市食品从业人员致泻大肠埃希菌感染、分布及毒力基因携带情况。方法抽取长沙市9个单位258例食品从业人员为研究对象，采用实时荧光多重聚合酶链反应（PCR）检测致泻大肠埃希菌。结果致泻大肠埃希菌感染率为15．5％（40／258），其中肠聚集性大肠埃希菌（EAEC）占10．9％（28／258），肠致病性大肠埃希菌（EPEC）占3．1％（8／258），肠出血性大肠埃希菌（EHEC）占1．6％（4／258），未检出肠侵袭性大肠埃希菌（EIEC）和肠产毒性大肠埃希菌（ETEC）。28株EAEC携带ui‐dA基因26株，aggR＋pic基因13株，astA基因27株，携带率分别为92．9％、46．4％、96．4％；8株EPEC全部携带eae和uidA基因；4株EHEC中3株携带eae基因，且都携带stx1＋stx2基因。结论建立持续监测致泻大肠埃希菌的机制和深入研究其分子流行病学理论，除了为监管部门制订和评价公共卫生措施提供基础数据，对评价食品安全状况，有效控制传染源、保护人民群众健康具有重要意义。
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