诺如病毒 GⅡ．4亚型新变异株全基因组序列的研究

摘要

目的：分析诺如病毒 GⅡ．4亚型新变异株的全基因组序列，了解其变异特点。方法从264例患者腹泻物中提取RNA 并进行 cDNA 合成，对阳性样本进行 PCR 鉴定，扩增片段进行测序。对测序结果显示为诺如病毒序列的进行全基因组测序并分析。结果分离到新的悉尼2012 GⅡ．4变异株的类似株（荆州2013 GⅡ．4株），全基因组序列测定发现新分离株出现了较大的变异，包括 VP1基因高变区（P2区）的突变。结论悉尼2012 GⅡ．4变异株的类似株已传播到中国，GⅡ．4变异株 VP1进化较快。提醒应密切关注诺如病毒在中国的传播和进化情况，以助于疫苗的开发和治疗性单抗的制备。%Objective Analysis of the complete genome sequence about the newly emerged pandemic norovirus GⅡ.4 genotype, to understand its variation characteristics.Methods 264 patients were collected with diarrhea.The RNA was extracted from 264 fe-cal specimens and cDNA was synthetized.The positive samples were amplified by PCR,the amplified fragments were sequenced. The complete genome sequences of the norovirus was sequenced and analyzed.Results A new norovirus variant strain of Jingzhou GⅡ.4,a pleiston of Sydney GⅡ.4 was isolated.A large variation was found in the new variant subtype,which was mutated inclu-ding in the hypervariable P2 domain of the major capsid protein VP1.Conclusion The result demonstrates the variant strain of Sydney GⅡ.4 was spread to China.VP1 of norovirus GⅡ.4 is evolving rapidly.The spread and evolution situation of the norovirus GⅡ.4 need to be closely monitored in China for the development of effective vaccines and therapeutic monoclonal antibodies.