夏枯草对AIT大鼠TSH、TGAb、TPOAb及Th相关细胞因子表达的调节作用研究

摘要

目的 通过研究夏枯草对自身免疫性甲状腺炎(AIT)大鼠促甲状腺激素(TSH)、甲状腺球蛋白抗体(TGAb)与甲状腺过氧化物酶抗体(TPOAb)及辅助性T 细胞(Th)相关细胞因子γ-干扰素(IFN-γ)、肿瘤坏死因子α(TNF-α)、白细胞介素4(IL-4)、白细胞介素17(IL-17)的调节作用,探讨夏枯草的作用机制,为临床运用提供理论基础.方法 将40只4～6周雌性SD 大鼠随机分为空白组、模型组、西药对照组(硒酵母组) 、夏枯草组.每组各10只,空白组予以常规饲养,模型及药物组大鼠予以猪甲状腺球蛋白(PT g )注射及高碘水喂养造模,造模后药物组大鼠分别予以硒酵母及夏枯草的生理水溶液灌胃,干预6周后处死大鼠,采用酶联免疫吸附试验(ELISA)法检测各组大鼠血清TSH 、TGAb、TPOAb、IFN-γ、TNF-α、IL-4、IL-17水平.结果 模型组大鼠TSH、TGAb、TPOAb、IFN-γ、TNF-α、IL-4、IL-17水平较空白组显著升高,差异有统计学意义(P＜0 .05).硒酵母组与夏枯草组TSH 、TGAb 、TPOAb 、IFN-γ 、TNF-α 、IL-4 、IL-17水平均低于模型组,差异有统计学意义(P＜0 .05) ;夏枯草组TSH 、IFN-γ 、IL-4 、IL-17水平均低于硒酵母组,差异有统计学意义(P＜0 .05).结论 夏枯草可以降低AIT大鼠TSH 、自身抗体及Th相关细胞因子的表达,具有免疫调节作用,值得在临床推广运用.%Objective To study and explore the effect of Prunella vulgaris on TSH 、TGAb 、TPOAb and Th cell-associated cytokines IFN-γ ,TNF-α ,IL-4 and IL-17 in autoimmune thyroiditis (AIT ) rats .Methods Di-vide forty female Sprague-Dawley rats into four group :blank group ,model group ,western medicine control group (selenium yeast group) ,Prunella vulgaris group ,10 rats in each group ,The rats in the model group and the rats in the model group were treated with PTg injection and high iodine water .The rats in the drug group were treated with sour yeast and Prunella vulgaris .The rats were sacrificed 6 weeks after the intervention . Elisa method was used to detect the rats Serum levels of TSH ,TGAb ,TPOAb and IFN-γ ,TNF-α ,IL-4 and IL-17 were measured .Results The levels of TSH ,TGAb ,TPOAb ,IFN-γ ,TNF-α ,IL-4 and IL-17 in the model group were significantly higher than those in the blank group ,the difference was statistically significant (P＜0 .05) .Selenium yeast group and Prunella vulgaris group TSH ,TGAb ,TPOAb ,IFN-γ ,TNF-α ,IL-4 ,IL-17 were lower than the model group ,the difference was statistically significant (P＜0 .05) ;The levels of TSH , IFN-γ ,IL-4 and IL-17 in Prunella vulgaris group were lower than those in selenium yeast group ,the difference was statistically significant (P＜0 .05) .Conclusion Prunella vulgaris can reduce the expression of autoanti-bodies and Th-related cytokines in AIT rats ,and have immunomodulatory effects ,it is worth to promote the use of clinical .