Regulatory effects of high glucose on the expression of Toll-like receptor gene and cytokine production in human peripheral-blood mononuclear cells

摘要

To investigate the regulatory effects of high glucose on the expression of Toll Like Receptor (TLR) Gene and the level of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in human peripheral-blood mononuclear cells. Methods The alterations of TLR1～10 mRNA expression in human peripheral-blood mononuclear cells were quantitated using real-time quantitative-polymerasechain reaction. The level of TNF-α and IL-6 were measured by Enzyme-Linked Immuno Sorbent Assay. The anti-TLRmAb were used to block the mononuclear cells 30 min, high glucose was used to stimulate the cells. Results high glucose strongly up-regulated the expression of TLR3, 5 mRNA but the expression of the other sub-TLRs weren't changed. The concentrations of TNF-α, IL-6 were 86.40±8.46 and 874.66±92.84 in high glucose group, all significantly higher than that the control experiment (60.49±6.80 and 541.80±81.08, all P<0.01), The anti-TLR3mAb and anti-TLR5mAb were used to block the mononuclear cells, The concentrations of TNF-α, IL-6 (72.41±8.52 and 700.59±84.88) were lower than that in the high glucose group, all P＜0.05. Conclusion high glucose may be endogenous ligand of TLRs and high glucose can regulate the release of inflammation cytokines from human peripheral-blood mononuclear cells through TLR signal way.