Objective To explore the expressions and significance of P-cadherin(P-ca),E-cadherin(E-ca), and β-catenin(β-ca)in colon polyps.Methods A total of 120 patients with colon polyps were selected and di-vided into the hyperplastic polyp group(40 cases),the tubular adenoma group(40 cases)and the villous adeno-ma group(40 cases).Other 20 healthy normal persons in the same period were selected as the control group. Immunohistochemistry(S-P)method was used to detect the expressions of P-ca,E-ca,and β-ca of subjects in paraffin sections of colonic polyp tissue and normal colonic mucosa.Western blotting semiquantitative method was used to detect the expression levels of P-ca,E-ca,and β-ca protein.Results P-ca was almost not express-ing in normal colonic mucosa,and positive signals in colon polyps tissue located in the cytoplasm and cell mem-brane,with the progression of the positive expression rate,it gradually increased(P<0.05).Positive-expres-sion of E-ca was in the cell membrane,with the progress of the disease,the positive-expression rate significant-ly decreased(P<0.05),β-ca expressed on the cell membrane in normal colonic mucosa tissue,with cytoplasmic of nuclear ectopic expression in the colon polyps group,with the progress of the disease,the positive rate of cytoplasm of nucleus gradually increased(P<0.05).Conclusion P-ca and β-ca show up-regulated expression in colon polyps,and E-ca show down-regulate expression,these three indicators were related closely to the oc-currence and development of colon polyps.%目的 探讨P钙黏蛋白(P-ca)、上皮性钙黏附因子(E-ca)、β-连环蛋白(β-ca)在结肠息肉中的表达及意义.方法 选取120例结肠息肉患者为研究对象,其中增生性息肉组、管状腺瘤组、绒毛状腺瘤组各40例,同期选取20例体检健康者纳入正常黏膜组,采用免疫组织化学(S-P)方法检测结肠组织石蜡切片中P-ca、E-ca及β-ca表达情况,采用免疫印迹试验检测P-ca、E-ca及β-ca蛋白表达水平.结果 P-ca在正常结肠黏膜组织中几乎不表达,在结肠息肉组织中阳性信号位于细胞膜及细胞质上,且随病情进展阳性表达率逐渐上升(P<0.05);E-ca阳性表达于细胞膜上,且随着病情进展阳性表达率呈明显下降趋势(P<0.05),β-ca在正常结肠黏膜组织中表达于细胞膜上,在结肠息肉组织中出现细胞质或细胞核异位表达,且随病情进展细胞质或细胞核阳性表达率逐渐上升(P<0.05).结论 P-ca、β-ca在结肠息肉中上调表达,E-ca下调表达,三者与结肠息肉的发生及发展紧密相关.
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