穿心莲内酯对脓毒症小鼠淋巴细胞免疫功能的调节作用

摘要

目的 探讨穿心莲内酯对脓毒症小鼠淋巴细胞免疫功能的影响.方法 采用盲肠结扎穿孔(cecal ligation andpuncture,CLP)制作脓毒症模型.48只C57BL/6小鼠按完全随机法分为4组,每组12只:假手术组(Sham组)、脓毒症对照组(CLP组)、二甲基亚砜(dimethyl sulfoxide,DMSO)组和穿心莲内酯组(AND组).各组小鼠术后1、6h和12h腹腔内分别注射等体积的生理盐水、DMSO或穿心莲内酯.术后24 h流式细胞仪检测脾脏CD4+T淋巴细胞、CD8+T淋巴细胞、CD19+B淋巴细胞以及CD11c+ DC细胞数量；流式细胞仪检测胸腺CD3+T淋巴细胞凋亡情况.结果 穿心莲内酯治疗后,小鼠脾脏CD4+T淋巴细胞、CD8+T淋巴细胞、CD19+B淋巴细胞以及CD1 1c+ DC计数分别为(8.03±0.62)×105/ml、(3.42±0.58)×105/ml、(6.48±1.13)×106/ml、(5.50±0.79)×106/ml,显著高于CLP组(P＜0.01)；治疗后的小鼠胸腺CD3+T淋巴细胞的凋亡明显减少(P＜0.01).结论 穿心莲内酯对脓毒症小鼠淋巴细胞免疫功能具有调节作用.%Objective To investigate the effects of andrographolide on the immune function of lymphocytes in mice with experimental sepsis.Methods Sepsis model was induced by cecal ligation and puncture (CLP).Forty eight C57BL/6 mice were randomly divided into 4 groups (n=12):sham group,CLP group,dimethyl-sulfoxide (DMSO) group and andrographolide (AND) group.Normal saline,DMSO and andrographolide were injected intraperitoneally in equal volume to each group respectively at 1,6 h and 12 h after surgery.The spleen and thymus of mice in each group were harvested 24 h after operation.CD4+ T,CD8+ T,CD19+ B lymphocytes,and CD1 1c+ dendritic cells (DCs) were assessed using fluorescence activated cell sorter in spleen.Apoptosis of CD3+ T lymphocytes was detected using fluorescence activated cell sorter.Results The counts of CD4+ T,CD8+ T,CD19+ B lymphocytes and DCs in the AND group were (8.03±0.62)×105/ml,(3.42±0.58)×105/m1,(6.48±1.13)×106/ml and (5.50±0.79)×106/ml,higher than those in the CLP group (P＜0.01).Apoptosis of CD3+ T cell was inhibited in thymus in the AND group compared to that in the CLP group(P＜0.01).Conclusions Andrographolide could modulate the immune function of lymphocytes in mice with CLP sepsis.