Suppression of novel andrographolide derivatives on murine splenic T lymphocyte proliferation

摘要

Andrographolide (1) is a primary constituent of Andrographis paniculata (Burm.f.) Nees which is a Chinese herbal medicine possesses extensive pharmacological effects.In this paper,the effect of compound 1 and some 15-alkylidene substituted derivatives of andrographolide (2-13) on murine splenic T lymphocyte proliferation reaction were appraised using MTT assay.The results showed that most of 15-alkylidene substituted andrographolides appraised could exert potent immunosuppressant effects.Especially compound 5,the most potent among all the tested compounds at concentrations with no obvious cytotoxicity,significantly inhibited the proliferation of murine splenic T lymphocytes activated by Con A in a dose-dependent manner (1.56μmol/l-12.50μmol/l),by the most inhibition of 55.8％.Findings implied that suitable 15-alkylidene substituted andrographolides might possess some better pharmacology activity than 1.