针刺对实验性脑出血大鼠脑组织bFGF表达的影响

摘要

目的:探讨急性脑出血后碱性成纤维细胞生长因子(bFGF)的动态表达及针刺的干预作用.方法:选取健康的雄性Wistar大鼠96只,随机分为模型组、模型加针刺组(针刺组)、模型加忆立福组(西药组)3组.每组又分为6h、24h、48h、72h和168h 5个时相点,每个时相点有6只大鼠,制备脑出血大鼠模型,采用Western免疫印迹技术观察头穴针刺治疗对脑出血bFGF的表达水平.结果:针刺组大鼠脑内bFGF蛋白表达在不同时相点均有显著上调,与模型组比较,均有显著性差异(P＜0.01),具有统计学意义.结论:上调脑内bFGF可能是有关针刺治疗脑出血一个机制,针刺可能通过使血肿周围脑组织内bFGF蛋白水平增加,从而减轻神经元损伤并促进神经修复与再生.%To study the dynamic expression of bFGF after acute intercerebral hemorrhage ( ICH ) in rats and the influence of acupuncture on it. Methods; The 96 healthy Wistar male rats were selected. The rats were divided into three groups randomly:model group, pinprick group and western medicine group,30 rats in each group. Each group was divided into five time phase points of 6 hours,24 hours,48 hours,72 hours and 168 hours,6 rats in each group. The experiment ICH model was made by injection antologous blood using ster-eotactic method.Another six rats were assigned as the matched blank group.We observed the expression of bFGF of the intercerebral hemorrage rats with the methods of western - blot. Results;The expressions of bFGF in pinprick group significantly up - regulated than those in the model group(P <0.01). Conclusion; Adjusting upwards expression of bFGF in brain may be one important mechanism.By intervention from early stage, acupuncture may adjust upwards expression of bFGF in the brain tissue around hematoma of rats with ICH so as to reduce the neuronal injury and promote nerve repair and regeneration.