Study on Effects of Acupuncture at the Heart Meridian on Gene Expression Pattern of Hypothalamus in Rats with Acute Myocardial Ischemia

摘要

Objective: To discovery the central mechanism of acupuncture Heart Meridian precondition myocardial ischemia in gene expression pattern,the authors applied gene chip tech to filter variably expressed genes in hypothalamus.Methods: Rats were seperated into normal,model,acupuncture Heart Meridian group and acup Lung Meridian group randomly.Acute myocardial ischemia rat model was made with ligation left anterior descending branch of the coronary artery.After model succeed,select hypothalamus seperately and mixed the same group together of 3 rats.Then applied Rats U230A genechip refered by Affymetrix Co.to compare the variations between these groups.Results:To compare with normal group,differential expression genes and expression sequence tags (ESTs) in model group were 73 with signal log ratio ≥ 1 and 92 with signal log ratio ≤-1 ,mainly included ion channel,calcium/calmodulin-dependent protein kinase Ⅱ inhibitor,antigen and so on.Similarly,compared withmodel group,differential expression genes and expression sequence tags (ESTs) were 190 with signal log ratio ≥ 1 and 34 with signal log ratio ≤-1 in acupuncture Heart Meridian group,mainly included 5-hydroxytryptamine receptor,cellular metabolism,fatty,immuno reaction,G-protein coupled receptors,ion transport,signal transductions and so on,while in acupucnture Lung Meridian,the number is 57 and 26 correspondly.Conclusion:There were exactlyvariations in hypothalamus mechanism that relate to acupuncture Heart and Lung Meridians.