新血府逐瘀汤对动脉粥样硬化模型大鼠免疫因子TGF-β、VCAM-1表达的影响

摘要

Objective:To observe the effect of New Xuefu Zhuyu decoction (NXFZY) on aorta pathological changes in AS rats and the expressions of TGF-βand VCAM-1,and to explore the possible mechanism of anti AS.Methods:40 SPF male Wistar rats were randomly divided into five groups which were a blank control group,a model group,a Liptor group,a low dose NXFZY group and a high dose NXFZY group.Except the control group, other groups copied AS models.In low dose NXFZY and high dose NXFZY groups, rats were intragastrically administered by NXFZY at the concentration of 9g/( kg·d) and 18g/( kg·d) respectively. In the Liptor group, rats were intragastrically administered by an aqueous solution of Liptor 2mg/( kg·d) . Simultaneously, the control and model groups were intragastrically administered with equal volume of normal saline.After eight weeks,thoracic aortic pathology changes in rats were observed by light microscope with HE staining.TGF-βand VCAM-1 expressions of thoracic aortic pathology was detected by immunohistochemi-cal method, while the correlativity was analyzed.Results:Compared with the model group, the NXFZY could increase the expression of TGF-β(P<0.01) and decrease the expression of VCAM-1(P<0.05),and the two had a significantly negative correlation(P<0.01).Conclusion:NXFZY can inhibit the inflammatory re-sponse by upregulating protective factor TGF-βexpression, thus achieving the effect of anti-atherosclerosis.%目的:观察新血府逐瘀汤对AS大鼠主动脉病理形态学改变及转化生长因子-β(TGF-β)、血管细胞黏附分子-1(VCAM-1)表达的影响,探讨新血府逐瘀汤抗动脉粥样硬化(AS)的可能机制.方法:健康雄性Wistar大鼠40只,随机分为对照组,模型组,立普妥组,新血府逐瘀汤低剂量组及新血府逐瘀汤高剂量组.除空白组外,其他各组复制AS模型,中药低剂量组9g/(kg·d)、中药高剂量组18g/(kg·d)灌胃;立普妥组2mg/(kg·d)水溶液灌胃;对照组及模型组灌服等容积生理盐水,连续8周;HE染色镜下观察胸主动脉病理学改变,免疫组织化学法检测胸主动脉TGF-β、VCAM-1表达,并进行相关性分析.结果:与模型组比较,新血府逐瘀汤高剂量组可显著提高TGF-β表达(P<0.01),降低VCAM-1表达(P<0.05),两者存在显著负相关性(P<0.01).结论:新血府逐瘀汤在一定程度上可通过上调保护性因子TGF-β以抑制免疫炎症反应,发挥抗动脉粥样硬化的作用.