新血府逐瘀汤对动脉粥样硬化模型大鼠主动脉形态及免疫调节因子HMGB-1、Foxp3表达的影响

摘要

目的:观察新血府逐瘀汤对动脉粥样硬化模型大鼠主动脉HMGB-1、Foxp3表达及病理形态学影响,探讨其调节免疫因子抗动脉粥样硬化的作用机制.方法:雄性SPF级Wistar大鼠48只,按随机数字表法分为空白对照组、模型组、立普妥组、新血府逐瘀汤(NXFZY)低剂量组、NXFZY高剂量组及NXFZY+立普妥组.除空白组外,其余各组复制动脉粥样硬化模型,NXFZY低剂量组新血府逐瘀汤9 g/(ks·d)、NXFZY高剂量组新血府逐瘀汤18g/(kg·d)灌胃,立普妥组给予阿托伐他汀钙2 mg/(kg·d)水溶液灌胃,对照组及模型组灌服等容积生理盐水,8周后HE染色后镜下观察胸主动脉病理学改变,免疫组织化学法检测胸主动脉HMGB-1、Foxp3表达并进行相关性分析.结果:与模型组比较,新血府逐瘀汤可显著降低HMGB-1表达,升高Foxp3表达,两者存在显著负相关性.结论:新血府逐瘀汤可通过下调促炎因子HMGB-1、上调抗炎因子Foxp3表达,调节AS过程中促炎与抗炎因子之间的平衡,发挥抗AS的作用.%Objective:By observing the effect of New Xuefu Zhuyu Decoction on aorta pathologymorphology and HMGB-1,Foxp3 expression in atherosclerosis model rats,explore its anti atherosclerosis mechanism by the regulation of immune factors.Methods:48 healthy male Wistar rats were randomly divided into blank control group,model group,western medicine group,low dose Chinese medicine group,high dose Chinese medicine group,and the group of high dose Chinese medicine plus western medicine.Except the blank group,other groups were copied to atherosclerosis model,low dose group and high dose group of Chinese medicine garaged with New Xuefu Zhuyu Decoction at a concentration of 9 g/ (kg · d) and 18g/ (kg · d) respectively;western medicine group with aqueous solution of atorvastatin calcium 2 mg/(kg · d);these two programs administered by adding for Integrative Medicine group;simultaneously,the control and the model group were fed with volume of normal saline.After 8 weeks,the thoracic aortic pathology change was observed under light microscope with HE staining;the HMGB-1,Foxp3 expression of thoracic aortic was detected by immunohistochemical method,and then their correlativity was analyzed.Results:Compared with the model group,the New Xuefu Zhuyu Decoction could decrease the expression of HMGB-1 and increase the expression of Foxp3,and the two have a significant negative correlation.Conclusion:New Xuefu Zhuyu Decoction could down regulate proinflammatory factor HMGB-1 and upregulation of anti-inflammatory factor Foxp3 expression,to play the role of anti atherosclerosis by regulating the balance of inflammatory factor.