金银花UPLC指纹图谱及其次生代谢物化学成分比较研究

摘要

Objective:To establish the fingerprint spectrum of Flos lonicerae by HPLC technique.Methods:With chlorogenic acid as reference, the fingerprint was established on Acquity UPLCTM BEH C18 Column (2.1 mm×50 mm, 1.7 μm).Acetonitrile-0.1% formic acid was used as gradient mobile phase at the flow rate of 0.3 mL/min.The column temperature was 40℃.The chromatograms of Flos lonicerae and its secondary metabolites were analyzed, and its quality was evaluated by Chinese Medicine Chromatographic Fingerprint Evaluation System (2004 A).Results: Common mode of the fingerprints was established from 10 batches of Flos lonicerae.There were total 10 common spectrum peaks taking chlonogenic acid as reference, which were identified with better separation.Conclusion: The method is time-saving, efficient and can be used for the quality control of Flos lonicerae.%目的:建立金银花的UPLC指纹图谱.方法:以绿原酸为参照物,采用Acquity UPLCTM BEH C18色谱柱(2.1 mm×50 mm,1.7 μm);流动相为乙腈-0.1%甲酸,梯度洗脱;流速为0.3 mL/min;柱温40℃.对金银花及其次生代谢物的色谱图进行分析,并利用《中药色谱指纹图谱相似度评价软件》(2004A)对二者共有峰的相似度进行质量评价.结果:建立了10批金银花的UPLC指纹图谱的共有模式,特征图谱有10个共有峰,以对照品绿原酸为对照,各色谱峰有较好的分离效果.结论:本方法快速、高效,可用于金银花药材的质量控制.