Objective To investigate region-specific reference ranges of peripheral blood lymphocyte subset percentages in he patitis B virus (HBV)-infected Chinese population. Methods Flow cytometry was used to detect the percentages of peripheral blood lymphocyte subsets in 2846 HBV-infected subjects and 117 healthy subjects in 302 Hospital of PLA from 2001 to 2009. The refe rence ranges of peripheral blood lymphocyte subset percentages in healthy and HBV-infected Chinese popula-tion were investigated. Results The reference ranges of the percentages of peripheral blood lymphocyte subsets including CD3+ T lymphocytes, CD3+CD4+ T lymphocytes, CD3+CD8+ T lymphocytes, CD4VCD8+ ratio, CD3-CD19+ B cells, CD3-CD16+CD56+ natural killer (NK) cells and CD3+ CD16+CD56+ NKT cells were defined in healthy adults and HBV-infected patients with acute hepatitis B, chronic hepatitis B(CHB), liver failure and liver cirrhosis (aged from 16 to 60 years old). It was found that the percentages of peripheral CD4+ T lymphocytes sig nificantly increased, and NK cells and NKT cells significantly decreased in HBV-infected adult patients as compared with healthy adults. Further correlation analysis indicated that the age of all the subjects was slightly positively associated with the percentages of pe ripheral CD4+ T lymphocytes, CD4/CD8 ratio, NK cells and NKT cells, but slightly negatively associated with CD8+ T lymphocytes and CD19+ B cells. Conclusions Through long-term and large-sample investigation, the region-specific reference ranges of peripheral blood lymphocyte subset percentages in HBV-infected adult Chinese patients are proposed. They will be helpful for the evaluation of immune status of the HBV-infected subjects in clinic.%目的 调查中国乙型肝炎患者外周血淋巴细胞亚群频率参考值范围.方法 利用流式细胞术检测2846例乙型肝炎患者和117例健康人群外周血淋巴细胞亚群数值,调查我国健康人群和乙型肝炎人群的参考值范围.结果 调查了16～60岁健康人群和HBV感染相关的急性肝炎、慢性肝炎、重型肝炎和肝硬化人群外周血CD3+T淋巴细胞、CD3+CD4+T淋巴细胞、CD3+CD8+T淋巴细胞、CD4+/CD8+比值、CD3-CD19+B细胞、CD3-CD16+CD56+自然杀伤细胞(natural killer cell,NK细胞)以及CD3+CD16+CD56+ NKT细胞亚群频率参考值范围.结果显示,HBV感染不同疾病进展人群外周血CD4+T淋巴细胞频率与成年健康人群比较显著升高,而NK和NKT细胞频率显著降低.还发现外周血CD4+T淋巴细胞、CD4+/CD8+比值、NK 以及NKT细胞亚群频率与年龄呈低度正相关,而CD8+T淋巴细胞和CD19+B细胞频率则与年龄呈低度负相关.结论 通过长期大样本调查,提出我国HBV感染的成年患者外周血淋巴细胞亚群频率参考值范围,为临床判断慢性乙型肝炎患者免疫状态提供参考依据.
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