急性乙型肝炎患者自然杀伤细胞的变化与疾病进展的关系

摘要

目的 探讨急性乙型肝炎(acute hepatitisB,AHB)患者外周血中自然杀伤细胞(natural killer cells,NK细胞)频率、功能变化及与疾病进展的关系.方法 入组15例AHB患者(AHB组)和14名健康者(健康对照组),采集外周血,用流式细胞仪分析NK细胞频率和活化指标,并与临床病毒学和生化指标进行相关性分析,同时观察NK细胞对K562细胞刺激的应答.结果 AHB组NK细胞频率低于健康对照组(P＜0.05).与健康对照组相比,AHB组NK细胞呈现高活化状态,且与转氨酶呈正相关.进一步研究发现,AHB组NK细胞分泌细胞因子IFNγ及脱颗粒活性(CD107a)的能力显著增强.结论 AHB组NK细胞高活化、分泌细胞因子能力和细胞毒能力显著增强,与临床指标有相关性,提示NK细胞在加速病毒清除的同时可引起肝损伤.%Objective To investigate the frequency and function of natural killer (NK) cells in peripheral blood in patients with acute hepatitis B (AHB) and their correlation with disease progression.Methods Peripheral blood samples from 15 AHB patients and 14 healthy controls were collected.The frequency and activation markers of NK cells were analyzed using flow cytometry and the correlations with serum ALT levels and HBV DNA levels were evaluated as well.Meanwhile,the response of NK cells to the stimulation of K562 cell line was observed.Results The frequency of NK cells in AHB patients was lower than that in healthy controls,and the difference between them was significant (P＜0.05).As compared with healthy controls,NK cells in AHB patients displayed higher activation,which was positively correlated with serum ALT levels.Furthermore,it was found that NK cells from AHB patients displayed an increased production of interferon (IFN) γand cytolytic activity (CD107a).Conclusions NK cells from AHB patients show increased activation and expression of IFN γ and cytotoxicity,which is correlated with clinical markers,suggesting NK ceils may cause liver injury while speeding up the viral clearance in AHB patients.