Genomic DNA was extracted from human blood using KI, primers were designed according to gene sequences in GenBank, then the potential interaction between different primers were analyzed. Combinations of different concentrations of primers, reaction buffer and Mg2+ were added to optimize the PCR system. Results showed when 1.4xPCR buffer, 2.5 mmol/L MgCU, 0.6 u,mol/L of each primer pair were in the 25 u,L reaction system, clear band with homogeneous luminance was amplified with anyone of the 3 pairs of primers.%采用碘化钾法从血液中提取基因组DNA,根据GenBank数据库中基因序列设计引物,分析不同引物组合内部可能出现的引物潜在配对作用,针对多重PCR反应体系中的引物、反应缓冲液和镁离子浓度设计实验组合.结果表明,25 μL反应体系中包含1.4×PCR buffer,2.5 mmol/L MgCl2,0.6 μmol/L的各引物对浓度时,3对引物都能扩增得到条带清晰,亮度均一的目标产物.
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