The establishment of an adenovirus-aprt vector system and the study of its application for human gene therapy.

摘要

The aim of this thesis research was to study the adenovirus as a vector for possible use in the human gene therapy. Recombinant adenovirus-5 vectors containing the Chinese hamster ovary cell (CHO) adenine phosphoribosyltransferase gene (aprt) have been constructed. Infection of aprt;There is an accumulation of antisense mRNA in the cells infected with the recombinant viruses in which the aprt gene was cloned in the opposite orientation to the viral E3 promoter. The presence of these antisense RNAs however did not significantly inhibit the APRT activity indicating that these RNAs are not responsible for the low APRT activity in Ad5/A1 and Ad5/PAL infected cells.;To test the possible use of the adenovirus vector for gene targeting, APRT