ELISA and the specific PCR of ALV-J were used to do serological detection and provirus nucleic acid diagnosis for 4 layers, which had clinical symptoms of that cockscombs were pale, bursa of feather and claw bleeded, phalang and xiphoid had tuomr, from a layer farms in Hubei province. As a result, provims nucleic acid diagnosis by ALV-J specific PCR and antigen detection from cloaca by ELISA were all positive of the 4 layers; but there was just one antibody positive by indrect ELISA. The results of serological detction and PCR test documented that the layers had infected with ALV-J and there were exists not only congenital infection or early infection, but also acquired infection in the layer flocks.%用ELISA和ALV-J特异性PCR对抽检的湖北省某蛋鸡场的4只鸡冠苍白、毛囊和鸡爪出血、脚趾间和剑状软骨处长有肿瘤的海兰褐病鸡,进行J亚群禽白血病血清学检测和ALV-J前病毒核酸检测.结果4只病鸡ALV-J特异性PCR检测和泄殖腔拭子ALV-J ELISA抗原检测全部为阳性;间接EUSA检测血清抗体,有3只鸡呈阴性,1只鸡为阳性.血清学检测和PCR检测表明,抽检病例为血管瘤型J亚群禽白血痛,并且鸡群中既存在先天感染或早期感染情况,也存在后天感染的现象.
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