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鸭源致病性大肠杆菌的分离鉴定及致病性观察

     

摘要

自湖北省荆州市某鸭场送检病料中分离到一株疑似鸭大肠杆菌,经选择性培养基培养、生化试验、PCR鉴定、动物回归试验、药物敏感性试验,确认为鸭大肠杆菌,该菌对多种抗生素具有耐药性,用已分离鉴定的大肠杆菌经腹腔注射途径感染15日龄雏鸭,致病性试验结果显示,经腹腔注射途径感染雏鸭死亡率达100%,引起纤维素性心包炎、肝被膜炎和气囊炎,说明此株大肠杆菌具有强致病性.%A suspected E. coli from ducklings was isolated in Jingzhou of Hubei province. After selective culturing, biochemical test. PCR identif'ication, regression test and medicine-resistance test, it was identif'ied to be Escherishia coli from ducklings with high resistance to several antibiotics. Subsequently, some 15 day-old ducklings were infected with the E. coli isolates by lumbar injection for pathogenicity test. The results showed that the mortality of infected ducklings was 100%. In the dead ducks fibrinous pericarditis, glissonities and air sacculitis were also ohserved. It proved that this E. coli isolate was in high pathogenicity.

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