抗噬菌体色氨酸酶基因工程菌的筛选

摘要

利用自发突变、紫外诱变、协同进化、紫外耦合协同进化4种方法对色氨酸酶基因工程菌WD0801进行了抗噬菌体筛选.结果表明紫外耦合协同进化法具有较好的筛选效果,筛选的42株稳定遗传的抗性菌株中有23株的长势高于出发株,11株的酶活优于出发株.%4 methods, spontaneous mutation,UV mutagenesis,co-evolution,UV coupled co-evolution, were employed to screen EscheTichia coli tryptophanase genetic engineering strains. The results showed that the screening efficiency of UV-coupled co-evolution method was the best. Among the 42 stable phage—resistant strains screened out, the growth vigor of 23 strains, and enzyme activity of 11 strains were higher than the original strain.