离心萃取-紫外光度法快速测定茶饮料中的咖啡因

摘要

建立以三氯甲烷为提取剂,以离心萃取为提取方法,以276 nm为检测波长的茶饮料中咖啡因的检测方法.结果表明,吸光度A与咖啡因浓度C在5.324～16.940 mg/L呈现良好的线性关系,相关系数为0.999 5,回收率为97.6％～99.4％.该方法测定快速、准确度高,适用于茶饮料中咖啡因的测定.%The UV-Vis method for detecting caffeine in tea drink at 276 nm after centrifugal extraction was established. The results showed that the absorbence (A) showed a good linear relation with concentration of caffeine (C) from 5.324 mg/L to 16.940 mg/L. The correlation coefficient was 0.999 5 and the recovery ranged from 97.6% to 99.4%. In conclusion, the method was suitable for rapid determination of caffeine in tea drink.