HPLC法检测鲟鱼中孔雀石绿、结晶紫及其代谢产物

摘要

High performance liquid chromatography(HPLC) method was used to detect malachite green,leucomalachite green,crystal violet and leucocrystal violet in sturgeons.The method of freezing was employed to remove oil in the samples,and the SPE column effect on the recovery of samples was discussed.The results showed that the recoveries were ranging from 61.24％ to 105.81％,and the relative standard deviation values for repeatability were 0.2％～6.3％.The detection limit of malachite green was 2 μg/kg,and the detection limits of leucomalachite green,crystal violet and leucocrystal violet were 1 μg/kg.This method is simple and can be used for fast analysis of a large number of samples.%采用高效液相色谱法同时检测鲟鱼(Acipenser sturio L.)中的孔雀石绿、隐色孔雀石绿、结晶紫和隐色结晶紫.采用冷冻的方法除去样品中所含的油脂,讨论了过柱与否对回收率的影响.结果表明,该方法测得的回收率为61.24％～105.81％,相对标准偏差为0.2％～6.3％,孔雀石绿的检出限为2 μg/kg,其他3种物质的检出限均为1 μg/kg.该方法处理简单,节省时间,可用于大量样品的快速分析.