猪TCAP基因转录因子的筛选与鉴定

摘要

In this study,1 662 bp sequence of pig TCA P gene promoter was cloned. Some specific transcription factor (MyoD, MyoG and MEF2) binding sites were found in TCA P gene promoter by bioinformatics analysis. Over-expression vectors of MyoD,MyoG and MEF2 were successfully constructed and transfected with promoter sequences, respectively. Detection analysis showed that TCA P promoter activity was significantly higher. The results indicated that transcription factors MyoD,MyoG and MEF2 could bind to the TCA P promoter sequences, and enhance its activity.%克隆了猪TCA P基因1662 bp的启动子序列,序列分析发现其启动子区域存在MyoD、MyoG、MEF2转录因子结合位点,并构建了3个转录因子超表达载体,分别与TCA P基因启动子载体共转染PK细胞。结果表明,3个转录因子使TCA P基因启动子活性升高,均正调控TCA P基因的表达。