小鼠血浆中白藜芦醇含量测定及药动学研究

摘要

Objective To develop a HPLC-UV method for quantitative determination of resveratrol in mice plasma and to study the pharmacokinetics of resvetrol in mice after oral administration. Methods Chromatographic separation was achieved on an Ultimate AQ- C18 column (150 mm×4.6 mm, 5 μn) using acetonitrile-water (32 : 68, V: V) as mobile phase at a flow rate of 1. 0 mL·min-1. The UV detection wavelength was set at 306 nm, and the assay was executed at 30℃. Results The method was validated and found to be linear in the range of 0. 10-10. 00 μg·mL-1. The assay accuracy and precision were within the range of 98. 0% - 102. 0%. The main pharmacokinetic parameters of resveratrol , Cmax was 3.589 μg· mL-1 ,tmax was 30 min, t1/2α was 10.5 min, t1/2β was 172.7 min. Conclusion The method was sensitive, simple and successfully applied in the study of pharmacokinetics of resveratrol in mice.%目的 建立测定小鼠血浆中白藜芦醇的高效液相色谱法,并研究白藜芦醇在小鼠体内的药动学.方法 色谱柱为C18柱(150 mm×4.6 mm,5μm),流动相乙腈-水(32∶68),流速为1.0 mL·min-1,检测波长306 nm,血浆样品用乙酸乙酯萃取法预处理.结果 白藜芦醇线性范围0.1 ～10.0μg·mL-1,r =0.9995.日内RSD＜10％,日间RSD＜15％,回收率98.0％～102.0％.小鼠灌服给予白藜芦醇主要药动学参数为30 min后血浆药物浓度达峰值,Cmax为3.589 μg·mL-1,t1/2α为10.5 min,t1/2β为172.7 min.结论 该方法灵敏度高,操作方便,适用于白藜芦醇在小鼠体内的药动学研究.