大孔树脂纯化柽柳总黄酮工艺的研究

摘要

Objective To study the conditions for isolating and purifying flavonoids in Tamarix chinensis Lour by D-101 type of niacroporous adsorption resin. Methods The total flavonoids in Tamarix chinensis Lour was taken as a reference index. The sample volume, adsorption time, eluting velocity, ethanol concentration and pH value of the sample solution were investigated. Results The optimum conditions for isolating and purifying flavonoids in Tamarix chinensis Lour by D-101 type of macroporous adsorption resin were as follows; pH = 6-7, 15 mL sample of the original liquid, adsorption time of 30 min, 70% ethanol as an eluting reagent, and an eluting velocity of 3 BV ? H-1. Conclusion D-101 macroporous adsorptive resin can effectively isolate and purify total flavones from Tamarix chinensis Lour.%目的 研究D-101大孔吸附树脂分离纯化柽柳总黄酮的工艺条件.方法 以柽柳总黄酮为指标,对上样量、吸附时间、洗脱速度、乙醇浓度和上样药液pH进行考察.结果 大孔树脂分离柽柳总黄酮的最佳工艺条件为上样量15 mL,pH 6～7,吸附30 min,流速3 BV·h-1,乙醇浓度70%,乙醇用量3 BV.结论 D-101大孔吸附树脂能有效分离纯化柽柳总黄酮.