蜗牛多糖对人肝癌细胞SMMC-7721的抑制作用

摘要

Objective To study the suppressive effect of snail polysaccharide on human hepatocellular carcinoma cell line SMMC-7721. Methods Human hepatocellular carcinoma cells SMMC-7721 were co-cultured with different dose of snail polysaccharide for 24, 48 and 72 h, respectively. The multiplication restrain on hepatic cancer cells by snail polysaccharide was determined by MTT method;and the cell cycle was detected by using flow cytometry. Results The absorbent value of cells affected under different concentrations of snail polysaccharide after 24, 48 and 72 h showed significant difference compared with the control at corresponding periods, ( P<0. 05 or P<0. 01 ) , and which presented time and dose related manner. The half inhibitory concentration ( IC50 ) of snail polysaccharide on SMMC-7721 cell strain was 0. 293, 0. 032 and 0. 011 mg · mL-1 , respectively. The result indicated that snail polysaccharide arrested SMMC-7721 the cell strain to G2/M by flow cytometry. Conclusion The snail polysaccharide inhibits proliferation of cell strain SMMC-7721.%目的 探讨蜗牛多糖对人肝癌细胞株SMMC-7721的抑制作用.方法 将不同浓度的蜗牛多糖分别与人肝癌细胞SMMC-7721共同培养24,48,72 h后,采用噻唑蓝法测定吸光度值(A),评定蜗牛多糖对肝癌细胞株的增殖抑制作用;流式细胞术测定其对肝癌细胞周期的影响.结果 不同浓度的蜗牛多糖作用SMMC-7721细胞24,48和72 h后,各组与细胞对照组比较,A值差异有统计学意义(P＜0.05或P＜0.01),并显示出对时间和剂量的依赖性,蜗牛多糖对SMMC-7721细胞的半数抑制浓度分别为0.293,0.032和0.011 mg·mL-1.流式细胞术结果表明,蜗牛多糖能将肝癌细胞株SMMC-7721阻滞在细胞的G2/M期.结论 蜗牛多糖对肝癌细胞株SMMC-7721的增殖有一定的抑制作用.