核桃楸皮提取物对人肝癌细胞SMMC-7721的增殖抑制作用及对细胞周期的影响

摘要

研究核桃楸皮提取物(EMBJM)对人肝癌细胞SMMC-7721的增殖抑制作用及对细胞周期的影响.体外培养SMMC-7721细胞,并用不同浓度核桃楸皮甲醇提取物处理.通过MTT法研究EMBJM对SMMC-7721细胞增殖活性的影响;采用流式细胞仪分析EMBJM对SMMC-7721细胞周期的影响.EMBJM可以显著抑制SMMC-7721细胞生长,具有时间和计量依赖性.细胞周期实验表明EMBJM将SMMC-7721细胞阻滞在G2/M和S期,并具有剂量依赖性.EMBJM质量浓度为8和16mg/L时,G2/M期细胞所占百分比分别为13.89％±2.46％和14.35％±2.68％,与空白对照组(8.34％±1.42％)比较,差异均有统计学意义(P＜0.05);同等浓度范围内S期细胞所占百分比分别为39.87％±2.83％和44.81％±2.93％,与空白对照组(26.80％±1.76％)比较,差异均有统计学意义(P＜0.05).桃楸皮甲醇提取物可以影响SMMC-7721细胞周期分布和有效抑制SMMC-7721细胞生长.%To study effect of extracts from bark of Juglans mandshurica (EMBJM) on proliferation and cell cycle of SMMC-7721 cells,the SMMC-7721 cells in culture medium in vitro were given different concentrations of EMBJM.The inhibition rate of EMBJM on the SMMC-7721 cell was detected by MTI assay.The cell cycle analyzed was detected by flow cytometry (FCM).The EMBJM exhibited a significant inhibition on SMMC7721 cells time-and dose-dependently.The cell cycle detection results suggested that EMBJM could arrest SMMC-7721 cells specifically in growth 2/mitosis(G2/M) and synthesis(S) phase cell cycle in a dose-dependent manner.Compared with control group (8.34％ ± 1.42％;26.80％ ± 1.76％),the G2/M and S phase of SMMC-7721 cells exposed to EMBJM (13.89％ ±2.46％,39.87％ ±2.83％ (8 mg/L);(14.35％ ± 2.68％,44.81％ ± 2.93％ (16 mg/L)) were significantly increased (P ＜0.05,n =3).EMBJM can effectively inhibited proliferation in SMMC-7721 cells and effected cell cycle distribution.