不同样品溶剂对高效液相色谱_蒸发光散射检测器测定黄芪甲苷的影响

摘要

Objective To exPlore the effect of samPle_solVent comPositions on the determination of AstragaliⅣby high Performance liquid chromatograPh_ eVaPoratiVe light scattering detector ( HPLC_ELSD ) . Methods Radix astragali and ganduqing granules serVed as samPles. Methanol,90%methanol,80%methanol,70%methanol,32%acetonitrile,15%acetonitrile, and water were samPle solVents. HPLC_ELSD was used to determine content of astragalosideⅣ. Results The results showed that the 90%methanol solution was an aPProPriate samPle solVent with good system suitability,Precision,accuracy,and,linearity etc. . Conclusion This method benefits the quality control of astragalosideⅣin Radix astragali and agents containing Radix astragali.%目的：考察不同样品溶剂对高效液相色谱_蒸发光散射检测器( HPLC_ELSD)测定黄芪甲苷的影响。方法以黄芪和感毒清颗粒为样品，以甲醇、90%甲醇、80%甲醇、70%甲醇、32%乙腈、15%乙腈和水为样品溶剂，采用HPLC_ELSD测定黄芪甲苷的含量。结果以90%甲醇溶液为样品溶剂，其系统适用性良好，准确度、精密度、线性关系等方法学验证指标符合要求。结论采用90%甲醇溶液作为样品溶剂，更有利于黄芪及制剂中黄芪甲苷的质量控制。