目的：探讨碱性成纤维细胞因子（ bFGF）调节皮肤创伤修复的作用机制。方法采用组织块贴壁法培养来源于正常皮肤和增生性瘢痕的成纤维细胞（FB），加入含有不同浓度bFGF（0，0.1，1，10，100，1000 ng·mL-1）的无血清培养液培养72 h，采用细胞计数法和锥虫蓝染色检测各组FB增殖和凋亡，酶联免疫吸附法（ ELISA）和逆转录PCR （ RT-PCR）分别检测Ⅰ型、Ⅲ型胶原和纤维连接蛋白浓度及基因表达情况。结果 bFGF促进FB增殖，当浓度过高时则抑制FB增殖，促进其凋亡，并且增生性瘢痕FB增殖较来源于正常皮肤的FB缓慢；bFGF明显抑制增生性瘢痕FB Ⅰ型胶原产生，对来源于正常皮肤FB无影响；bFGF上调来源于正常皮肤FB的纤维连接蛋白基因表达，对增生性瘢痕FB中的表达无影响；bFGF对两种来源的FBⅢ型胶原的分泌和表达均无显著作用。结论 bFGF对来源正常皮肤和增生性瘢痕的FB具有不同的影响和作用机制，可能对创伤修复早期和瘢痕形成过程发挥作用。%Objective To explore the mechanism and effects of basic fibroblast growth factor( bFGF)on skin wound healing. Methods Fibroblasts( FB)were isolated from normal skin and hypertrophic scar and cultivated by direct adherence method. FB were then treated with different concentrations of bFGF(0,0. 1,1,10,100,1 000 ng·mL-1 )and cultivated with serum-free medium for 72 hours. The proliferation and apoptosis of FB in each group were detected by cell counting and trypan blue staining. Content and gene expression of typeⅠand type Ⅲ collagen and fibronectin were determined by ELISA and RT-PCR,respectively. Results bFGF promoted the proliferation of FB at low concentrations promoted apoptosis of FB at higher concentrations. The proliferation of FB from hypertrophic scar was slower than that from the normal skin. bFGF significantly inhibited type Ⅰ collagen production from hypertrophic scar FB but not from the normal skin. Moreover,bFGF up-regulated fibronectin expression in the normal fibroblasts,but not in the hypertrophic scar. No change in type Ⅲ collagen expression and production was observed in FB from either source. Conclusion bFGF has differential effects and mechanisms on FB of the normal skin and hypertrophic scar,suggesting that bFGF may play a role in early phase of skin wound healing and scar formation.
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