Objective To observe the effects of Xifeng Tongnao capsules on the content of TNF-α and IL-1β in brain tissue of rats with focal cerebral ischemia-reperfusion injury. Methods A total of 120 adult SD rats were randomized into 6 groups: Xifeng Tongnao low-, middle- and high-dose groups, model control group, Buchang Naoxintong group and sham-operated group.Buchang Naoxintong group were treated with Buchang Naoxintong capsules at 0.864 g��kg-1.Xifeng Tongnao high-, middle-and low-dose groups were treated with 3.456, 1.728, and 0.864 g��kg-1 Xifeng Tongnao capsules, respectively;sham-operated group and model control group were treated with equal volume of purified water. Medications were administered intragastrically once daily for 7 days. The acute transient focal cerebral ischemia-reperfusion model was established by middle cerebral artery occlusion ( MCAO) 1 h after the final dose, and rats in the sham-operated group only received anesthesia and stripping without occlusion.All rats were sacrificed after reperfusion for 24 h, and expression levels of TNF-α and IL-1β in the brain tissue were detected by ELISA. Results TNF-αcontent in Xifeng Tongnao capsutes low-, middle-and high-dose groups were (35.34±8.95), (33.75±6.92), and (40.95±5.39) ng��L-1, respectively.IL-1β content were (1.44±0.47), (1.45± 0.23), and (1.61±0.33) ng��L-1 in low-, middle- and high-dose groups, respectively.TNF-α and IL-1β were (38.96±9.84) and (1.56±0.31) ng��L-1, respectively in Buchang Naoxintong group, (52.74±6.76) and (2.79±0.45) ng��L-1in the model control group, and (32.54±4.00) and (1.32±0.22) ng��L-1 in sham-operated group.TNF-αand IL-1βcontent were significantly higher in Buchang Naoxintong group than in sham-operated group ( P<0. 05 ) . TNF-α and IL-1β content were significantly decreased in Xifeng Tongnao high-, middle- and low-dose groups (all P<0.05). Conclusion Xifeng Tongnao capsules can protect brain tissue by supressing TNF-α and IL-1β and alleviating inflammatory injury from ischemia.%目的：探讨熄风通脑胶囊对局灶性脑缺血-再灌注损伤大鼠脑组织肿瘤坏死因子-α( TNF-α)和白细胞介素-1β( IL-1β)含量的影响。方法健康斯泼累格��多雷( SD)大鼠120只，随机分为6组，假手术组、模型对照组，步长脑心通组，熄风通脑胶囊大剂量组，中剂量组和小剂量组。各组大鼠造模前进行预防性给药，步长脑心通组给予步长脑心通0.864 g��kg－1，熄风通脑胶囊大剂量组、中剂量组和小剂量组分别给予熄风通脑胶囊内容物3.456，1.720，0.864 g��kg－1，假手术组、模型对照组给予等体积纯化水，连续灌胃给药7 d，每天1次。末次给药后1 h，采用改良线栓法( MACO)制备大鼠局灶性脑缺血-再灌注损伤模型，假手术组只麻醉剥离不插入线栓；24 h后取脑，用酶联免疫法检测大鼠脑组织中TNF-α、IL-1β的含量。结果熄风通脑胶囊大剂量组、中剂量组和小剂量组TNF-α分别为(35.34±8.95)，(33.75±6.92)，(40.95±5.39)ng��L－1，IL-1β分别为(1.44±0.47)，(1.45±0.23)，(1.61±0.33) ng��L－1，步长脑心通组TNF-α、IL-1β分别为(38.96±9.84)和(1.56±0.31) ng��L－1，模型对照组分别为(52.74±6.76)和(2.79±0.45) ng��L－1，假手术组分别为(32.54±4.00)和(1.32±0.22) ng��L－1。模型对照组大鼠血清中 TNF-α、IL-1β含量均明显高于假手术组(P<0.05)。熄风通脑胶囊大剂量组、中剂量组和小剂量组大鼠血清中TNF-α、IL-1β含量降低(均P<0.05)。结论熄风通脑胶囊可通过抑制脑组织TNF-α、IL-1β的升高，减轻缺血时炎性损伤，对脑缺血大鼠脑组织产生一定程度的保护作用。
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