不同产地及不同药用部位马蓝中靛蓝和靛玉红的含量测定

摘要

Objective To establish a RP-HPLC method for determining indigo and indirubin in Baphicacanthus cusia from different producing areas and medicinal parts. Methods The separation was achieved by an Agilent TC-C18 Column (4.6 mm×250 mm, 5 μm) at 25 ℃ using methanol-water (75��25) as mobile phase at a flow rate of 1 mL��min-1.The detection wavelength was 290 nm. Results Indigo had a good linear relationship with peak area at range of 0. 051 3-0.820 8 μg (r=0.999 3).The recovery rate was 99.00% and RSD was 1.30% (n=6).Indirubin had a good linear relationship with peak area at range of 0.049 5-0.792 0 μg (r=0.999 9).The recovery rate was 98.88% and RSD was 1.51% (n=6). Conclusion The contents of the two components are obviously different in Baphicacanthus cusia because of different places or medicinal parts. The proposed method is simple, rapid and reliable. This method for determination of indigo and indirubin in Baphicacanthus cusia by RP-HPLC provides a basis for quality control of Baphicacanthus cusia.%目的：建立反相高效液相色谱( RP-HPLC)法同时测定不同产地、不同药用部位马蓝中靛蓝、靛玉红的含量。方法选用 Agilent TC-C18色谱柱(4.6 mm ×250 mm，5μm )，流动相为甲醇-水(75��25)，柱温25℃，流速1.0 mL��min－1，检测波长290 nm。结果靛蓝在0.0513~0.8208μg范围内与峰面积呈良好的线性关系(r＝0.9993)，平均回收率为99.00％，RSD为1.30％(n＝6)。靛玉红在0.0495~0.7920μg范围内与峰面积呈良好的线性关系(r＝0.9999)，平均回收率为98.88％，RSD为1.51％( n ＝6)。结论马蓝中靛蓝、靛玉红因产地和药用部位的不同，含量差异较大。该方法操作简便、快速、可靠，可为马蓝药材的质量控制提供实验依据。