不同产地黄药子中黄独乙素的含量测定

摘要

目的：建立高效液相色谱( HPLC)法测定不同产地黄药子中黄独乙素的含量并比较其差异。方法药材粉末用纯净水直接煎二次,合并水煎液,采用Kromasil-C18(4.6 mm×250 mm,5μm )色谱柱,以乙腈水醋酸=34660.1为流动相,流速为1.0 mL·min-1,检测波长210 nm,柱温35℃。结果黄独乙素在26.0~130.0μg·mL-1范围内线性关系良好(r=0.9999),不同产地黄药子中黄独乙素平均含量：河北>广西>江苏>四川>浙江。结论该方法操作简便、快捷,结果准确、可靠,适用于不同产地黄药子中黄独乙素的含量测定。%Objective To establish a HPLC method for determining and comparing the contents of diosbulbin B in Dioscorea bulbifera L. from different regions. Methods The medicine powders were extracted twice by water, then the water extracts were combined and detected by HPLC at 35℃,with Kromasil-C18 column (4. 6 mm×250 mm,5μm) and a mobile phase of acetonitrile-water-glacial-acetic acid (34660. 1). The flow rate was 1. 0 mL·min-1 and the detection wavelength was 210 nm. Results The linear range of diosbulbin B was 26. 0-260. 0 μg·mL-1(r=0. 999 9). The average content of diosbulbin B in Dioscorea bulbifera L. from different areas was that of Hebei>Guangxi>Jiangsu>Sichuan>Zhejiang. Conclusion The method is simple,quick,accurate and suitable for the determination of diosbulbin B in Dioscorea bulbifera L. from different regions.