表没食子儿茶素没食子酸酯联用顺铂对结肠腺癌细胞株SW620增殖和凋亡的影响

摘要

Objective To investigate the effect of different composed combination of EGCG and cisplatin on human colon adenocarcinoma cell line SW620 and the related mechanisms. Methods SW620 cells at exponential phase were treated with cisplatin(5 mmol.L-1)and EGCG(0, 25, 50 and 100 mmol.L-1).Seventy hours after the cultivation, cell proliferation was detected with MTT;the percentage of apoptotic cells was determined by flow cytometry;expression levels of JAK2, p-JAK2, p-STAT3, STAT3, Bax, Bcl-2, cleavage-PARP, and cleavage-caspase3 were detected by Western blotting. Results The SW620 cell proliferation rate was(95±5)%,(67±5)%,(42±5)%(22±4)% and the apoptotic rat was 5%, 15%, 35%, 57%in the 0, 25,50, 100 mmol.L-1 EGCG groups, respectively.Moreover, EGCG up-regulated the expression of Bax, cleavage-PARP and cleavage-caspase3(P<0.05)and down-regulated the expression of p-JAK2, p-STAT3 and Bcl-2(P<0.05)in a dose-dependent manner, but had no influence on the expression of JAK2 and STAT3(P>0.05). Conclusion EGCG can promote cisplatin-induced apoptosis and proliferation inhibition of human colon adenocarcinoma cell lines of SW620 by suppressing JAK2/STAT3 gignaling pathway.%目的：探讨表没食子儿茶素没食子酸酯( EGCG)和顺铂联用对结肠腺癌细胞株SW620的作用及其机制。方法5 mmol.L－1顺铂＋0,25,50,100 mmol.L－1 EGCG处理对数生长期的 SW620细胞。细胞培养72 h 后,噻唑蓝( MTT)法检测细胞增殖；流式细胞法检测细胞凋亡,免疫印迹法检测细胞 JAK2、p-JAK2、p-STAT3、STAT3、Bax、Bcl-2、cleavage-PARP、cleavage-caspase3表达。结果0,25,50,100 mmol.L－1 EGCG组 SW620细胞增殖率分别为(95±5)％,(67±5)％,(42±5)％,(22±4)％,细胞凋亡率分别为5％,15％,35％,57％,剂量依赖性上调 Bax、cleavage-PARP 和cleavage-caspase3表达( P<0.05),下调p-JAK2、p-STAT3和Bcl-2( P<0.05),对JAK2和STAT3表达无明显影响( P>0.05)。结论 EGCG可通过抑制JAK2/STAT3信号通路而增强顺铂诱导SW620细胞增殖抑制和凋亡。