河南省小麦赤霉病禾谷镰刀菌的毒素化学型及SCAR类型分析

摘要

To identify the mycotoxin chemotype and SCAR in Fusarium graminearum of winter wheat in Henan areas. The diseased heads of wheat heads blight are widely sampled in the main areas of wheat production in Henan. A total of 47 F. graminearum isolates are separated. The result of detection by using mycotoxin chemotype primers is that 27 F. graminearum isolates are 15-AcDON chemotype(57.45%),and 19 F. graminearum isolates are 3-AcDON chemotype(40.43%),while only one F. graminearum isolate is the NIV chemotype(2.13%). The 15-AcDON chemotype isolates are SCARⅠ. The 3-AcDON and NIV chemotype isolates are SCARⅤ. It suggestes that the 15-AcDON toxin producer is the dominant population,and the 3-AcDON toxin producer is the minor population,while the NIV toxin producer is very low.%为了弄清河南地区小麦赤霉病致病禾谷镰刀菌所产的毒素化学型和SCAR类型,对我国小麦主产区河南地区的小麦赤霉病病穗进行了广泛采样,共分离出47株禾谷镰刀菌菌株.经镰刀菌毒素特异性引物测定,27株菌株产15-AcDON型毒素,占分离出的禾谷镰刀菌菌株的57.45%;19株菌株产3-AcDON型毒素,占40.43%;仅有1株菌株产NIV型毒素,占2.13%.其中,产15-AcDON型毒素的菌株属于SCARⅠ类型,产3-AcDON和NIV型毒素的菌株属于SCARⅤ类型.结果表明,河南省产15-AcDON型毒素的禾谷镰刀菌是优势种群,产3-AcDON型毒素的禾谷镰刀菌种群次之,产NIV型毒素的禾谷镰刀菌种群所占比例很低.