酸性神经肽1对小鼠大脑氨基酸类神经rn递质及cGMP影响的实验研究

摘要

Objective： The aim of the research is to probe the changes of some neurotransmitters of amino acid (glutamic acid,Glu)and (γ-aminobutyric acid,GABA)and cGMP in mice brains caused by bovine acidic neuropeptide 1(BANP-1）．Methods: Mice were separated into six groups (n=15 at each group).Five of the groups were injected with BANP 1 into mice's abdominal cavities at dose of 15 mg/kg and brain was collected and homogenated at 0.5 h，1 h,2 h,3 h and 4 h for examination.The other group was injected with normal saline as control group and brain was collected at 1 h for examination.Brain Glu and GABA were analysised by filter paper-electrophoresis method.Brain cGMP were analysised by radiation immunity method.Results： After injection of BANP-1 in 1 h, 2 h,3 h,4 h,the results were compared with that of control group.It indicated that:Glu concentration had a significant increase in 1 h,2 h(P＜0.01，P＜0.01 respectively).GABA also increased in 2 h,3 h(P＜0.01 respectively).cGMP concentration increased in 1 h，2 h(P＜0.05，P＜0.01 respectively)．Conclusion: After injection of BANP-1，the concentrations of Glu and GABA and cGMP increased at different time.%目的： 探讨酸性神经肽(bovine acidic neuropeptide 1 BANP-1）对小鼠脑内氨基酸类递质(谷氨酸和γ-氨基丁酸)及cGMP的影响。方法： 将小鼠随机分为6组(每组15只)，其中5组按15 mg/kg腹腔注射BANP-1，将小鼠在注射后0.5 h，1 h，2 h，3 h，4 h处死并取脑匀浆，另一组为注射生理盐水对照组，在注射盐水后1 h处死并取脑匀浆，用75%冰乙醇抽提氨基酸类物质及cGMP并沉淀蛋白质。应用滤纸电泳法对小鼠大脑谷氨酸(glutamic acid,Glu)和γ-氨基丁酸(γ-aminobutyric acid,GABA)进行测定，应用放射免疫法对小鼠大脑环磷酸鸟苷(cyclic monophosphate guanosine,cGMP)进行测定。结果： 腹腔注射BANP-1后，各项指标在不同时间的结果分别与对照组比较：Glu浓度在1 h、2 h时明显高于对照组(P＜0.01)，0.5 h、3 h、4 h时差异无显著性(P＞0.05)。GABA浓度在2 h、3 h时显著高于对照组(P＜0.01)，0.5 h、1 h、4 h时差异无显著性(P＞0.01)。cGMP浓度有一定升高，1 h 2 h时显著升高(P＜0.01)，其余各时间组差异无显著性(P＞0.05)。结论： BANP-1急性用药后在一定时间可升高脑内Glu、GABA及cGMP水平。