益生菌制剂对肝硬化患者机体免疫功能的影响分析

摘要

Objective To investigate the influence of probiotics on immune function of cirrhosis patients and toxin level and cell factorin serum.Methods 36 cases with cirrhosis were regarded as subjects,conventional drugs and probiotics administration lasted for fourweeks were given,and the use of flow cytometry in patients before and after treatment was detected,to observe patients′peripheral T totalnumber of cells,as well as subsets of the level of natural killer cells.Azo chromogenic substrate method and enzyme -linked immunosorbentassay in combination with measurement of serum cytokine was used to detect cell factor in patients′serum: interleukin (i.e.,IL) -1β,IL -10,TNF levels in the plasma and toxins.Results T cells of patients with cirrhosis of peripheral (i.e.,CD3) and total CD4 (P <0.05) were measured significantly lower than normal levels.The use of probiotics after treatment,patients and subsets in vivo CD3 CD4levels were significantly increased (P <0.05); and serum IL -1β,IL -10,TNF levels were significantly improved (P <0.05).Conclusion Probiotics on immune dysfunction in patients with improvement was effective,safe and reliable.It can be applied in the clinical promotionas a treatment method for the treatment of chronic liver disease.%目的：探讨益生菌对肝硬化患者的机体免疫功能以及血浆中内毒素水平和血清中细胞因子的影响。方法选择本院36例肝硬化患者作为实验对象，给予常规的护肝保肝药物以及益生菌制剂，给药时间持续4周，使用流式细胞术在用药前后对患者进行检测，观察患者外周T细胞总数、亚群情况以及其自然杀伤细胞的水平。将偶氮基质显色法与酶联免疫吸附法测定结合使用测定患者血清中细胞因子：白细胞介素（即IL）－1β、IL－10、TNF和血浆中内毒素的水平。结果经测定肝硬化患者体内外周T细胞（即CD3）以及CD4总数明显低于正常水平（P＜0．05），采用益生菌制剂治疗后，患者体内CD3和CD4的亚群水平明显升高（P＜0．05）；患者血清中IL－1β、IL－10、TNF的水平显著改善（P＜0．05）。结论益生菌制剂对患者免疫功能紊乱有改善作用，且疗效确切，安全可靠，在临床中可以将其推广作为治疗慢性肝病的一种治疗方法。