里氏木霉纤维素酶生产工艺的优化

摘要

提高纤维素酶生产效率,降低纤维素酶生产成本是纤维素乙醇生产技术的关键之一。而碳源、氮源和无机盐等产酶培养基成分以及接种时间、产酶温度、培养初始pH等产酶条件是纤维素酶生产过程中的关键因素。为充分利用里氏木霉生产纤维素酶,研究了纤维素酶高产菌株里氏木酶FST-1产酶培养基和产酶条件对纤维素酶产酶的影响。结果表明：麸皮、蛋白胨和磷酸二氢钾的含量对于纤维素酶的生产影响较大,并且确定了最优产酶培养基为4号培养基。通过对不同产酶条件的研究,确定最佳接种时间为24h、最佳产酶温度为32℃、最佳初始pH为5.5,优化后的生产工艺可以将滤纸酶活力和蛋白含量提高3倍。%Reducing cellulose production cost through improving cellulose titers during the fermentation is a key strategy for developing economically competitive bioethanol production from lignocellulosic biomass.Medium composition and inoculate time,temperature,initial pH and other factors were key factors to cellulose production.For the purpose to fully used Trichoderma reesei to produce cellulose,the influence of medium composition and growth condition of the cellulose-producing strain Trichoderma reesei FST-1on its enzyme production was investigated.The result showed that the contents of wheat bran,peptone and KH 2 PO 4 had the significant effect on cellulose titer,the No.4medium was the best medium.Through the study on different enzyme production condition,it found that the optimum inoculation time was 24hours,the optimum temperature was 32℃and the optimum initial pH was 5.5.Cellulose titer and soluble protein content were increased 3times with optimized culture condition.