HPLC-UV 法测定草决明中五种蒽醌类化合物含量及其指纹图谱的建立

摘要

目的：建立一种同时测定草决明中五种蒽醌类化合物含量的HPLC方法及草决明HPLC指纹图谱。方法：采用Hypersil C18色谱柱（4．6mm×250mm，5μm），以乙腈-0．05％甲酸水溶液为流动相，梯度洗脱；流速1．0mL/min，柱温：30℃，检测波长254nm。采用评价软件（2004A）进行数据分析，对来自加纳和中国13个地区，共22种生、炒草决明建立了HPLC 指纹图谱，并进行相似度计算。结果：方法的灵敏度、线性、精密度以及准确度均符合方法学验证的要求。分别在生品中检出16个共有峰，在炮制品检出8个共有峰；与对照样品（加纳的生品）相比，中国的两个样本相似系数分别为0．577（南京）和0．565（武汉）。结论：所建立的草决明指纹图谱的方法稳定可靠，适用于草决明质量评价，两个国家的草决明质量有明显差异。%Objectiev : To establish a simultaneous determination of five cassia anthraquinones content by HPLC , and HPLC fingerprint method of grass cassia .M ethod:Hypersil C18 column (4.6 mm ×250 mm, 5μm), acetonitrile -0.05%formic acid as a mobile phase .The flow rate was 1.0 mL/min, the temperature was 30℃, and the detection wave length was 254 nm.Using chromatographic fingerprint evaluation software ( 2004 A) for data analysis , from Ghana and China , established HPLC fingerprint and similarity calculation .Result: The sensitivity, linearity , precision and accuracy of the method are in line with the requirements of method validation .16 and 8 common peaks were detected in crude drug and processed one respec-tively,compared with the control sample ( Ghana raw goods ) , similar coefficients of two samples from China were 0.577 ( Nanjing ) and 0.565 ( Wuhan ) , indicating significant difference between two areas of grass Cassia in quality .Conclusion:Cassia HPLC fingerprint method established stable and reliable , and suitable for quality evaluation Cassia herbs .