不同频率的周期性压力对兔软骨细胞影响的生物学研究

摘要

Objective:By observing cyclical pressures of different frequencies on rabbit chondrocyte proliferation and se -cretion of integrin α1β1, to study the effects and mechanism on rabbit chondrocytes .Method:Isolated fetal rabbit articular chondrocytes , the third-generation cells were seeded in plates .Set control group A ( normal culture ) and group B of different frequencies pressure (pressure 172kPa, frequency group B1 0.2Hz, group B2 0.5Hz, group B3 1Hz, group B4 2Hz, group B5 5Hz) .Pressure intervention of different frequencies 2 hours per day , 3 consecutive days after the intervention , to detect the proliferation of chondrocytes by Optical Density ( OD) , and ELISA assay was used to measure the integrin α1β1 level of culture supernatant.Result:OD value of group A was 1.08 ±0.13, group B1 was 1.19 ±0.11, group B2 was 1.23 ±0.08, group B3 was 1.35 ±0.06, group B4 was 1.45 ±0.09, group B5 was 1.37 ±0.12.Pressure groups compared with the control group was increased, the difference was statistically significant .There is significant difference between groups B , the peak appeared in B4 group.Group A of integrin α1β1 level was 3.02 ±0.26 pg/mL, group B1 was 3.28 ±0.19 pg/mL, group B2 was 3.43 ±0.11 pg/mL, group B3 was 3.77 ±0.24pg/mL, group B4 was 4.01 ±0.09 pg/mL, group B5 was 3.94 ±0.13 pg/mL.Integrinα1β1 value of pressure groups compared with the control group was increased , the difference was statistically significant ( P<0. 05) .There was significant difference between groups B; the peak appeared in group B 4.Conclusion: The same size ( 172 kPa) cyclic pressure stimuli on rabbit chondrocytes at different frequencies can promote chondrocyte proliferation and increase the integrins , but not the higher the frequency , the stronger the effect.In this study, the frequency of 2Hz has the strongest effect.%目的：通过观察不同频率的周期性压力对兔软骨细胞增殖及整合素α1β1分泌量的影响，研究不同频率的力学刺激对软骨细胞产生的生物学影响。方法：取兔四肢关节的软骨细胞，在体外进行培养。表型稳定的第3代细胞进行研究，设置常压培养A组和周期性压力B组，并将B组在压力为172kPa按照加压频率分组，加压频率B1组为0．2Hz，B两组0．5Hz ，B3组1Hz，B4组2Hz，B5组5Hz。 B组每天干预2h，连续干预3d。检测A和B组软骨细胞增殖情况和整合素α1β1的含量。结果：OD值A组为1．08±0．13，B1组为1．19±0．11，B两组为1．23±0．08，B3组为1．35±0．06，B4组为1．45±0．09，B5组为1．37±0．12。加压各组与对照组比较OD值升高，差异有统计学意义。 B组各组间差异有统计学意义，峰值出现在B4组。 A组整合素α1β1水平为3．02±0．26pg/mL，B1组为3．28±0．19pg/mL， B两组为3．43±0．11pg/mL，B3组为3．77±0．24pg/mL，B4组为4．01±00．9 pg/mL，B5组为3．94±0．13pg/mL。加压各组与对照组比较整合素α1β1含量均有升高，差异有统计学意义差异（ P＜0．05）。 B组各组间整合素差异有统计学意义，峰值出现在B4组。结论：相同大小（172kPa）不同频率的周期性压力刺激兔软骨细胞，均能促进软骨细胞增殖和增加整合素含量的作用，但并非频率越高作用越强。本实验中以2Hz频率的周期性压力作用最强。