目的：探讨在髁突软骨细胞受到周期性单轴压力后，结缔组织生长因子（connective tissue growth factor，CTGF）表达的影响，为正畸治疗中髁突软骨受力后的改建提供生物学依据。方法选取1周龄SD大鼠，提取并培养髁突软骨细胞，免疫组化鉴定。利用四点弯曲细胞力学加载仪对第3代细胞进行力值为2000u strain、0.5Hz的体外周期性单轴压力加载，分别在加力0min、30min、60min和120min后继续培养24h，应用蛋白印迹法检测在不同加力时间CTGF蛋白表达的变化。应用SPSS18.0软件对数据进行统计学分析。结果 CTGF的相对蛋白量在加力0min、30min、60min和120min后，分别为0、1.59、2.34和3.16，随着加载时间的增加，表达呈逐渐上升趋势。且组间差异具有统计学意义（P＜0.05）结论周期性单轴压力可刺激大鼠髁突软骨细胞CTGF的表达。%Object To investigate the effect of cyclic compressive stress on the expression of connective tissue growth factor (CTGF) in rat mandibular condylar Chondrocytes. Methods Condyle chondrocytes were isolated from rat condylar cartilage. The third generation of Mandibulae Condyle chondrocytes were subjected to 0.5Hz frequency with 2000u strain strength for 0, 30, 60 and 120 min using a Four Point Bending system. The cells were then collected after 24 hours. The expression of CTGF was detected by western blot. All data obtained were analyzed by SPSS 18.0. Results The relative amount of protein CTGF after 0 min, 30 min, 60 min and 120 min of cyclic compressive stress loading were respectively 0, 1.59, 2.34, and 3.16. With the increasing time of cyclic compressive stress loading, the expression of CTGF increased. There were significant differences among those groups (P<0.05). Conclusions Cyclic Compressive Stress induced the increased expression of CTGF in rat mandibular condylar Chondrocytes. As a cytokine, CTGF may mediate the morphological changes by cyclic compressive stress in condylar cartilage.
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