KW-2478对肿瘤细胞增殖作用的影响

摘要

目的:研究KW-2478对Eca109和KYSE-150食管癌细胞株、SGC-7901胃癌细胞株、HepG2肝癌细胞株、MCF-7乳腺癌细胞株5种肿瘤细胞体外增殖作用的影响,同时观察KW-2478对HL-7702正常肝细胞的作用.方法:体外培养HL-7702,Eca109,KYSE-150,SGC-7901,MCF-7和HepG2细胞,给予不同浓度的KW-2478,24h和48h后采用四甲基偶氮唑盐微量酶反应比色法(MTT法)检测细胞增殖情况.结果:不同浓度KW-2478作用于肿瘤细胞24h和48h后,5种肿瘤细胞的增殖能力均降低,呈现剂量和时间依赖性,且在Eca109细胞和HepG2细胞呈现剂量与时间交互作用,但HL-7702细胞的增殖情况未受明显影响.结论:KW-2478对Eca109,KYSE-150,SGC-7901,MCF-7和HepG2细胞有抑制作用,对HL-7702正常肝细胞的增殖无明显影响.%Objective:To study the effect on proliferation of KW-2478 to Eca109 and KYSE-150 e-sophageal cancer cell line, SGC-7901 gastric cancer cell line, HepG2 hepatoma cell line and MCF-7 breast cancer cell in vitro. And to observe the effect of KW-2478 to HL-7702 normal hepatocytes. Methods:HL-7702, Eca109, KYSE-150, SGC-7901, MCF-7 and HepG2 cell lines were cultured in vitro, give different concentrations of KW-2478, MTT assay test cell proliferation after 24h and 48h. Results:After 24h and 48h different concentrations of KW-2478 to the tumor cells, the proliferation of five tumor cells were reduced in dose-dependent and time-dependent, and showed dose and time interaction on Eca109 cells and HepG2 cells, but HL-7702 was not significantly affected. Conclusion:KW-2478 have inhibitory effect to Eca109, KYSE-150, SGC-7901, MCF-7 and HepG2 cells, and had no significant effect on the proliferation of HL-7702 normal hepatocytes.