目的 检测肝癌(HCC)患者组织中IGF-Ⅱ、血管内皮生长因子 (VEGF)的表达及临床意义.方法 采用免疫组织化学染色方法 检测HCC及癌旁组织中IGF-Ⅱ、VEGF蛋白表达水平.结果 HCC及癌旁组织IGF-Ⅱ蛋白阳性表达率分别为55%(22/40)、12.5%(5/40),VEGF蛋白阳性表达率分别为62.5%(25/40)、12.5%(5/40),HCC组织中IGF-Ⅱ、VEGF蛋白表达均显著高于癌旁组织(P<0.05),且IGF-Ⅱ、VEGF表达呈明显正相关(r=0.59,P<0.05).IGF-Ⅱ、VEGF表达与患者的年龄、性别、肿瘤大小无关,但与肿瘤的组织学分级、是否侵及包膜及有无癌栓密切相关.结论 与癌旁组织比较,HCC组织中IGF-Ⅱ、VEGF呈现高表达,提示IGF-Ⅱ、VEGF参与了HCC的发生发展过程,可能与HCC的生长和增殖有关.%Objective To investigate the expression and significance of IGF- II and VEGF in tissues of hepatocellular carcinoma ( HCC ). Methods The expression of IGF- II and VEGF in HCC tissues were detected by means of immunohistochemistry. Results The expression rates of IGF- II in HCC and tumor-adjacent tissues were 55% ( 22/40 ),12.5%( 5/40 ), respevtively, and the expression rates of VEGF were 62. 5% ( 25/40 ), 12. 5% ( 5/40 ), respectively. The expression rates of IGF- II and VEGF were significantly higher than those in tumor-adjacent tissues ( P <0. 05 ), furthermore, the expression of IGF- II was positively correlated with that of VEGF ( r = 0. 59, P < 0. 05 ). The expression of IGF- II and VEGF protein was not related with patients' sex, age or tumor' s size,however,which was closely correlated with histological grade,capsule violation and cancer embolus of tumors( P <0.05 ). Conclusion The overexpression of IGF- II and VEGF in HCC tissues may participate in the pathogenesis and progression of HCC,which may be correlated with the growth and proliferation of HCC.
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