病毒性心肌炎脂联素表达及替米沙坦的干预效果

摘要

Objective To investigate the effect of telmisartan on the expression levels of adiponectin,tumor necrosis factor-α( TNF-α) in mice with viral myocarditis.Methods The animal models with viral myocarditis were induced by Coxsackie B3 virus in 60 mice in which 30 mice were given telmisartan by gavage from the day of viral infection for 7 days ( observation group ) ,however,the other 30 mice without drug intervention were served as control group,and the other 10 mice without viral infection and without drug intervention were served as blank control group.The myocardial pathological changes and pathological score were observed,and the levels of serum adiponectin and TNF-αwere detected for three groups by double antibody sandwich enzyme-linked immune method.Results The serum levels of adiponectin in observation group were significantly higher than those of control group,however,the serum levels of TNF-αand pathological score were obviously lower than those in control group ( P <0.05).As compared with those in blank control group,the levels of adiponectin,TNF-α, pathological score in observation group were significantly increased ( P <0.05).As compared with those in blank control group,the serum levels of adiponectin in control group were significantly decreased,however,the levels of TNF-α,pathological score were significantly increased ( P <0.05 ) .The myocardial edema, inflammatory cells infiltration, myocardial cell degeneration and necrosis were found in control group at 7 days after viral infection,however,these changes were obviously. Conclusion Telmisartan can increase the expression levels of adiponectin having protective effects on heart,and can decrease the levels of TNF-α,thus which can relieve myocardial injury.%目的：探讨替米沙坦对病毒性心肌炎小鼠脂联素，肿瘤坏死因子-α表达的影响。方法制备柯萨奇B3病毒感染的心肌炎小鼠60例，其中30例自病毒感染之日并连续7 d喂食替米沙坦作为观察组；另外30例无药物干预的心肌炎小鼠作为对照组；10例未感染病毒也无药物干预的小鼠作为空白组。观察3组小鼠的心肌病理组织学积分；组织病理改变；双抗体夹心酶联免疫法测定血清脂联素、TNF-α浓度。结果喂食替米沙坦的观察组血清脂联素水平明显高于未药物干预的对照组，而TNF-α、病理积分均低于对照组，差异有统计学意义（ P ＜0．05）。与空白组相比，观察组血清脂联素、TNF-α、病理积分水平明显升高，差异有统计学意义（ P ＜0．05）。与空白组相比，对照组血清脂联素降低，而TNF-α、病理积分水平明显升高，差异有统计学意义（ P ＜0．05）。对照组小鼠接种病毒后第7天，可见心肌水肿、炎性细胞浸润、心肌细胞变性坏死等，观察组心肌炎症细胞较对照组明显减少。结论替米沙坦能够增加对心脏有保护作用的脂联素表达，减少TNF-α的分泌，减少心肌炎症损伤。