自发性高血压大鼠血清脂联素和心肌脂联素受体1的表达及胰岛素抵抗程度与心室重构的关系研究

摘要

Objective To observe the expression of serum adiponectin hormone ( APN ), myocardial adiponectin receptor 1 ( AdipoR1 ), the degree of insulin resistance ( IR ) in spontaneously hypertensive rats ( SHR ), to explore their relationship to elevation of blood pressure and ventricular remodeling. Methods The serum APN, fasting insulin ( FINS ) were determined in 22 - week - old SHR ( SHR group, n = 8 ) and Wistar - Kyoto rats ( WKY group, n = 8 ) . The distribution of myocardial AdipoR1 and its protein expression were detected by immunohistochemical method, AdipoR1 mRNA by real - time PCR. Results As compared with WKY group, BW reduced, SBP, HW, LVW, HWI, LVWI, FINS and HOMA - IR increased in SHR group, the difference was significant ( P < 0. 05 ), but there was not difference in FBG ( P > 0. 05 ) . There was difference in APN expression between 2 groups ( P < 0. 05 ) . The AdipoR1 positive staining decreased in SHR group, differing from WKY group ( P <0. 05 ) . AdipoR1 nRNA expression was lower in SHR group than in WKY group, the difference was significant ( P < 0. 05 ) .By Pearson linear correlation, SBP was negatively correlated with APN , AdipoR1 protein, AdipoR1 mRNA , positively with HWI, LVWI, HOME - IR; By multiple linear regression analysis, AdipoR1 protein, AdipoR1 mRNA, HWI, SBP were influencing factors of LVWI. Conclusion SHR has obvious ventricular remodeling, serum APN and AdipoR1 expression reduce; SHR ventricular remodeling may be correlated with the reduction of serum APN and AdipoR1 expression.%目的 观察自发性高血压大鼠(SHR)血清脂联素(APN)、心肌脂联素受体1(AdipoR1)的表达及胰岛素抵抗(IR)程度,探讨3者与血压升高、心室重构的关系.方法 选取22周龄SHR和Wistar-Kyoto(WKY)大鼠各8只(SHR组、WKY组)为研究对象,测定血清APN、血清空腹胰岛素(FINS),免疫组化法测定心肌AdipoR1的分布及蛋白表达,实时荧光定量聚合酶链式反应(PCR)法测定心肌AdipoR1 mRNA表达.结果 (1)与同周龄WKY组相比,SHR组体质量(BW)减低,收缩压(SBP)、全心重量(HW)、左心室重量(LVW)、心脏重量指数(HWI)、左心室重量指数(LVWI)、空腹胰岛素(FINS)及胰岛素抵抗指数(HOMA-IR)增高,差异均有统计学意义(P＜0.05);两组FBG差异无统计学意义(P＞0.05).(2)与WKY组相比,SHR组血清APN表达降低,差异有统计学意义(P＜0.05).(3)SHR组心肌AdipoR1阳性染色减少,与WKY组比较差异有统计学意义(P＜0.05).(4)SHR组心肌AdipoR1 mRNA表达低于WKY组,两组比较差异有统计学意义(P＜0.05).(5)Pearson直线相关显示,大鼠SBP与血清APN、AdipoR1、AdipoR1 mRNA呈负相关,与HWI、LVWI、HOMA-IR呈正相关;多元线性逐步回归分析显示,血清APN和HOMA-IR是大鼠SBP的影响因素.(6)Pearson直线相关显示,大鼠LVWI与血清APN、AdipoR1、AdipoR1 mRNA呈负相关,与SBP、HWI、HOMA-IR呈正相关;多元线性逐步回归分析显示,心肌AdipoR1、心肌AdipoR1 mRNA、HWI、SBP是大鼠LVWI的影响因素.结论 SHR存在明显的心室重构,血清APN及心肌AdipoR1表达下降、IR程度增加;SHR心室重构可能与血清APN及心肌AdipoR 1表达下降有关.