Liquid chromatography method of high performance for the determination of l-glycine ethyl benzene Deng sylvite is established. C18 column is adopted with the mobile phase of 0. 01 mol/L phosphate buffer (containing 0. 2% triethylamine. Adjusted pH to 3.0 by 85% phosphoric acid) -acetonitrile's (5 ' 95.V/V). The detection wavelength is 220 nm. L-glycine ethyl benzene Deng sylvite hydrolyzed at pH 2. 0. Then phenylglycine is used as the external standard to calculate the content. A good linearity is shown in the range of 2 ~ 20 mg/mL (r=0. 999 9), and the recoveries are 96%~98%.%建立了测定左旋苯甘氨酸乙基邓钾盐含量的高效液相色谱方法.采用Thermos C18色谱柱,流动相为0.01 mol/L磷酸盐缓冲液(含0.2％(体积分数)三乙胺,85％(体积分数)磷酸,调节pH值为3.0)-乙腈(体积比为5∶95)为流动相,检测波长为220 nm.样品在pH值为2.0条件下水解后,以水解产物苯甘氨酸为外标,计算左旋苯甘氨酸乙基邓钾盐的含量.左旋苯甘氨酸乙基邓钾盐质量浓度在2～20 mg/mL范围内线性关系良好(r=0.999 9),回收率为96％～98％.
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