盐酸阿霉素在 balb/c 小鼠各脏器浓度的 HPLC-荧光定量分析方法的建立

摘要

Objective To formulate a quantitative analysis method of HPLC with fluorescence detector for analyzing the concentration of Doxorubicin in vivo in balb/c mice.Method The mobile phase has been investigated to study the possibility of daunorubicin hydrochloride as the internal standard substance with a fluorescence detector .Organic solvents were used to extract the drugs in the organ-homogenates of female balb/c mice.The recovery rate, the precision, and stability of the samples were inspected under the conditions .Findings Standard curves were successfully obtained where the R2 value was between 0.9991 and 0.9998.The recovery rate was between 90%and 110%.The precision and the stability of the samples were found desirable .Conclusion The method we formulated was found to be simple , feasible, repeatable and accurate .It can be applicable to biological samples'analysis .%目的：建立盐酸阿霉素的balb／c小鼠体内HPLC-荧光分析方法。方法采用荧光检测器，选择最佳流动相，考察盐酸柔红霉素作为内标物的可能性；使用雌性balb／c小鼠为实验动物，取出脏器匀浆，以有机溶剂提取匀浆液中的药物；并对此条件下的回收率、精密度和以及样品稳定性进行考察。结果制备的标准曲线R2值在0．9991～0．9998，回收率基本保持在90％～110％，精密度及稳定性良好。结论本文建立的分析方法简便、可行，重复性、准确性较好，适用于生物样品的体内分析。